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Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos

Methyl green has long been known as a histological stain with a specific affinity for DNA, although its fluorescent properties have remained unexplored until recently. In this article, we illustrate the method for preparing a methyl green aqueous stock solution, that when diluted can be used as a ve...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Prieto, Daniel, Aparicio, Gonzalo, Machado, Matías, Zolessi, Flavio R.
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4542129/
https://ncbi.nlm.nih.gov/pubmed/25993383
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52769
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