High Efficacy but Low Potency of δ-Opioid Receptor-G Protein Coupling in Brij-58-Treated, Low-Density Plasma Membrane Fragments

PRINCIPAL FINDINGS: HEK293 cells stably expressing PTX-insensitive δ-opioid receptor-Gi1α (C(351)I) fusion protein were homogenized, treated with low concentrations of non-ionic detergent Brij-58 at 0°C and fractionated by flotation in sucrose density gradient. In optimum range of detergent concentr...

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Vydáno v:PLoS One
Hlavní autoři: Roubalova, Lenka, Vosahlikova, Miroslava, Brejchova, Jana, Sykora, Jan, Rudajev, Vladimir, Svoboda, Petr
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2015
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Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4540457/
https://ncbi.nlm.nih.gov/pubmed/26285205
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0135664
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