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Requirement of a polypyrimidine tract for trans-splicing in trypanosomes: discriminating the PARP promoter from the immediately adjacent 3' splice acceptor site.

We studied sequence requirements for trans-splicing at the 3' splice acceptor site of a procyclic acidic repetitive protein (PARP) coding gene in trypanosomes. In transient CAT transfection assays with linker scanning (LS) mutants in a PARP promoter--3' splice acceptor site--CAT construct, minor dif...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:EMBO J
Hauptverfasser: Huang, J, Van der Ploeg, L H
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group 1991
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC453125/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1935907/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1991.tb04957.x
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