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Studying Lipolysis in Adipocytes by Combining siRNA Knockdown and Adenovirus-Mediated Overexpression Approaches

3T3-L1 adipocytes are widely used as a model system for studying hormone-stimulated lipolysis. However, these cells were limited in their utility for gain- and loss-of-function studies due to the low efficiency of their transfection with plasmid DNA or small interfering RNA (siRNA) oligos. In this c...

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Bibliographic Details
Published in:Methods Cell Biol
Main Authors: Zhang, Xiaodong, Heckmann, Bradlee L., Liu, Jun
Format: Artigo
Language:Inglês
Published: 2013
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4529287/
https://ncbi.nlm.nih.gov/pubmed/24099289
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-408051-5.00006-1
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