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Data analysis as a source of variability of the HLA-peptide multimer assay: from manual gating to automated recognition of cell clusters

Multiparameter flow cytometry is an indispensable method for assessing antigen-specific T cells in basic research and cancer immunotherapy. Proficiency panels have shown that cell sample processing, test protocols and data analysis may all contribute to the variability of the results obtained by lab...

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Dettagli Bibliografici
Pubblicato in:Cancer Immunol Immunother
Autori principali: Gouttefangeas, Cécile, Chan, Cliburn, Attig, Sebastian, Køllgaard, Tania T., Rammensee, Hans-Georg, Stevanović, Stefan, Wernet, Dorothee, thor Straten, Per, Welters, Marij J. P., Ottensmeier, Christian, van der Burg, Sjoerd H., Britten, Cedrik M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Springer Berlin Heidelberg 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4528367/
https://ncbi.nlm.nih.gov/pubmed/25854580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00262-014-1649-1
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