Data analysis as a source of variability of the HLA-peptide multimer assay: from manual gating to automated recognition of cell clusters

Multiparameter flow cytometry is an indispensable method for assessing antigen-specific T cells in basic research and cancer immunotherapy. Proficiency panels have shown that cell sample processing, test protocols and data analysis may all contribute to the variability of the results obtained by lab...

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Detalhes bibliográficos
Publicado no:Cancer Immunol Immunother
Main Authors: Gouttefangeas, Cécile, Chan, Cliburn, Attig, Sebastian, Køllgaard, Tania T., Rammensee, Hans-Georg, Stevanović, Stefan, Wernet, Dorothee, thor Straten, Per, Welters, Marij J. P., Ottensmeier, Christian, van der Burg, Sjoerd H., Britten, Cedrik M.
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Berlin Heidelberg 2015
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Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4528367/
https://ncbi.nlm.nih.gov/pubmed/25854580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00262-014-1649-1
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