Data analysis as a source of variability of the HLA-peptide multimer assay: from manual gating to automated recognition of cell clusters

Multiparameter flow cytometry is an indispensable method for assessing antigen-specific T cells in basic research and cancer immunotherapy. Proficiency panels have shown that cell sample processing, test protocols and data analysis may all contribute to the variability of the results obtained by lab...

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Publicado en:Cancer Immunol Immunother
Autores principales: Gouttefangeas, Cécile, Chan, Cliburn, Attig, Sebastian, Køllgaard, Tania T., Rammensee, Hans-Georg, Stevanović, Stefan, Wernet, Dorothee, thor Straten, Per, Welters, Marij J. P., Ottensmeier, Christian, van der Burg, Sjoerd H., Britten, Cedrik M.
Formato: Artigo
Lenguaje:Inglês
Publicado: Springer Berlin Heidelberg 2015
Materias:
Original Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4528367/
https://ncbi.nlm.nih.gov/pubmed/25854580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00262-014-1649-1
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