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Retention of the Native Epigenome in Purified Mammalian Chromatin
A protocol is presented for the isolation of native mammalian chromatin as fibers of 25–250 nucleosomes under conditions that preserve the natural epigenetic signature. The material is composed almost exclusively of histones and DNA and conforms to the structure expected by electron microscopy. All...
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4527833/ https://ncbi.nlm.nih.gov/pubmed/26248330 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0133246 |
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