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The yeast branchpoint sequence is not required for the formation of a stable U1 snRNA-pre-mRNA complex and is recognized in the absence of U2 snRNA.

Commitment complexes contain U1 snRNP as well as pre-mRNA and are the earliest functional complexes that have been described during in vitro spliceosome assembly. We have used a gel retardation assay to analyze the role of the yeast pre-mRNA cis-acting sequences in commitment complex formation. The...

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Bibliografiske detaljer
Udgivet i:EMBO J
Principais autores: Séraphin, B, Rosbash, M
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 1991
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC452775/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1827069/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1991.tb08062.x
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