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Localization of the active site of human tumour necrosis factor (hTNF) by mutational analysis.
In order to define the active site(s) of human tumour necrosis factor (hTNF), we mutagenized its gene at random and directly screened the resulting population for loss of cytotoxic activity on L929 cells. Four biologically inactive mutant proteins (Arg32----Trp, Leu36----Phe, Ser86----Phe and Ala84-...
Gorde:
| Argitaratua izan da: | EMBO J |
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| Egile Nagusiak: | , , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
Nature Publishing Group
1991
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC452723/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2009860/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1991.tb08015.x |
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