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The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.
We have used site-directed mutagenesis to change amino acid side chains that have been shown crystallographically to be in close proximity to a DNA 3' terminus bound at the 3'-5' exonuclease active site of Klenow fragment. Exonuclease assays of the resulting mutant proteins indicate t...
Tallennettuna:
| Julkaisussa: | EMBO J |
|---|---|
| Päätekijät: | , , |
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
Nature Publishing Group
1991
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC452606/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1989882/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1991.tb07916.x |
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