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Systematic Mutagenesis of the Escherichia coli Genome
A high-throughput method has been developed for the systematic mutagenesis of the Escherichia coli genome. The system is based on in vitro transposition of a modified Tn5 element, the Sce-poson, into linear fragments of each open reading frame. The transposon introduces both positive (kanamycin resi...
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| 發表在: | J Bacteriol |
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| Main Authors: | , , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Microbiology (ASM)
2004
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC451658/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15262929/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.186.15.4921-4930.2004 |
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