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Systematic Mutagenesis of the Escherichia coli Genome
A high-throughput method has been developed for the systematic mutagenesis of the Escherichia coli genome. The system is based on in vitro transposition of a modified Tn5 element, the Sce-poson, into linear fragments of each open reading frame. The transposon introduces both positive (kanamycin resi...
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| Hauptverfasser: | , , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology
2004
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC451658/ https://ncbi.nlm.nih.gov/pubmed/15262929 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.186.15.4921-4930.2004 |
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