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Functional Characterization of the Mannitol Promoter of Pseudomonas fluorescens DSM 50106 and Its Application for a Mannitol-Inducible Expression System for Pseudomonas putida KT2440

A new pBBR1MCS-2-derived vector containing the Pseudomonas fluorescens DSM10506 mannitol promoter P(mtlE) and mtlR encoding its AraC/XylS type transcriptional activator was constructed and optimized for low basal expression. Mannitol, arabitol, and glucitol-inducible gene expression was demonstrated...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Hoffmann, Jana, Altenbuchner, Josef
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4514859/
https://ncbi.nlm.nih.gov/pubmed/26207762
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0133248
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