Nested PCR Biases in Interpreting Microbial Community Structure in 16S rRNA Gene Sequence Datasets

BACKGROUND: Sequencing of the PCR-amplified 16S rRNA gene has become a common approach to microbial community investigations in the fields of human health and environmental sciences. This approach, however, is difficult when the amount of DNA is too low to be amplified by standard PCR. Nested PCR ca...

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Veröffentlicht in:PLoS One
Hauptverfasser: Yu, Guoqin, Fadrosh, Doug, Goedert, James J., Ravel, Jacques, Goldstein, Alisa M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2015
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Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4509648/
https://ncbi.nlm.nih.gov/pubmed/26196512
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0132253
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