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An Efficient Genome-Wide Fusion Partner Screening System for Secretion of Recombinant Proteins in Yeast

To produce rarely secreted recombinant proteins in the yeast Saccharomyces cerevisiae, we developed a novel genome-wide optimal translational fusion partner (TFP) screening system that involves recruitment of an optimal secretion signal and fusion partner. A TFP library was constructed from a genomi...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Bae, Jung-Hoon, Hyun Sung, Bong, Kim, Hyun-Jin, Park, Soon-Ho, Lim, Kwang-Mook, Kim, Mi-Jin, Lee, Cho-Ryong, Sohn, Jung-Hoon
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4508530/
https://ncbi.nlm.nih.gov/pubmed/26195161
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep12229
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