Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses

Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Chan, Jasper Fuk-Woo, Choi, Garnet Kwan-Yue, Tsang, Alan Ka-Lun, Tee, Kah-Meng, Lam, Ho-Yin, Yip, Cyril Chik-Yan, To, Kelvin Kai-Wang, Cheng, Vincent Chi-Chung, Yeung, Man-Lung, Lau, Susanna Kar-Pui, Woo, Patrick Chiu-Yat, Chan, Kwok-Hung, Tang, Bone Siu-Fai, Yuen, Kwok-Yung
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2015
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4508434/
https://ncbi.nlm.nih.gov/pubmed/26019210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01224-15
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