Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion

To further our aim of synthesizing aldehyde-tagged proteins for research and biotechnology applications, we developed methods for recombinant production of aerobic formylglycine-generating enzyme (FGE) in good yield. We then optimized the FGE biocatalytic reaction conditions for conversion of cystei...

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Bibliografski detalji
Izdano u:J Biol Chem
Glavni autori: Holder, Patrick G., Jones, Lesley C., Drake, Penelope M., Barfield, Robyn M., Bañas, Stefanie, de Hart, Gregory W., Baker, Jeanne, Rabuka, David
Format: Artigo
Jezik:Inglês
Izdano: American Society for Biochemistry and Molecular Biology 2015
Teme:
Enzymology
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4505483/
https://ncbi.nlm.nih.gov/pubmed/25931126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.652669
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