Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion

To further our aim of synthesizing aldehyde-tagged proteins for research and biotechnology applications, we developed methods for recombinant production of aerobic formylglycine-generating enzyme (FGE) in good yield. We then optimized the FGE biocatalytic reaction conditions for conversion of cystei...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:J Biol Chem
Hlavní autoři: Holder, Patrick G., Jones, Lesley C., Drake, Penelope M., Barfield, Robyn M., Bañas, Stefanie, de Hart, Gregory W., Baker, Jeanne, Rabuka, David
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Biochemistry and Molecular Biology 2015
Témata:
Enzymology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4505483/
https://ncbi.nlm.nih.gov/pubmed/25931126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.652669
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky