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Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion
To further our aim of synthesizing aldehyde-tagged proteins for research and biotechnology applications, we developed methods for recombinant production of aerobic formylglycine-generating enzyme (FGE) in good yield. We then optimized the FGE biocatalytic reaction conditions for conversion of cystei...
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| Publicado no: | J Biol Chem |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4505483/ https://ncbi.nlm.nih.gov/pubmed/25931126 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.652669 |
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