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Back to Basics – The Influence of DNA Extraction and Primer Choice on Phylogenetic Analysis of Activated Sludge Communities

DNA extraction and primer choice have a large effect on the observed community structure in all microbial amplicon sequencing analyses. Although the biases are well known, no comprehensive analysis has been conducted in activated sludge communities. In this study we systematically explored the impac...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Albertsen, Mads, Karst, Søren M., Ziegler, Anja S., Kirkegaard, Rasmus H., Nielsen, Per H.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4504704/
https://ncbi.nlm.nih.gov/pubmed/26182345
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0132783
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