Isolation of GD3 synthase gene by expression cloning of GM3 alpha-2,8-sialyltransferase cDNA using anti-GD2 monoclonal antibody.

For the isolation of ganglioside GD3 synthase (EC 2.4.99.8) cDNA, we developed an expression cloning approach that used an anti-GD2 monoclonal antibody for selection. A host recipient cell line that we have named KF3027-Hyg5 was also utilized. This cell line expresses high levels of GM2 as well as G...

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Main Authors: Haraguchi, M, Yamashiro, S, Yamamoto, A, Furukawa, K, Takamiya, K, Lloyd, K O, Shiku, H
Format: Artigo
Jezik:Inglês
Izdano: 1994
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC45039/
https://ncbi.nlm.nih.gov/pubmed/7937974
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