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Mapping the rRNA neighborhood of the acceptor end of tRNA in the ribosome.

In order to map the rRNA environment of the acceptor end of tRNA in th e ribosome, hydroxyl radicals were generated in situ from Fe(II) attached via an EDTA linker to the 5' end of tRNA. Nucleotides in rRNA cleaved by the radicals were identified by primer extension, and assigned to the ribosom...

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Detalles Bibliográficos
Autores principales: Joseph, S, Noller, H F
Formato: Artigo
Lenguaje:Inglês
Publicado: 1996
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC450288/
https://ncbi.nlm.nih.gov/pubmed/8631311
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