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Dumbbell-PCR: a method to quantify specific small RNA variants with a single nucleotide resolution at terminal sequences

Recent advances in next-generation sequencing technologies have revealed that cellular functional RNAs are not always expressed as single entities with fixed terminal sequences but as multiple isoforms bearing complex heterogeneity in both length and terminal sequences, such as isomiRs, the isoforms...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Honda, Shozo, Kirino, Yohei
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4499115/
https://ncbi.nlm.nih.gov/pubmed/25779041
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv218
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