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Purification of high quality RNA from synthetic, polyethylene glycol based hydrogels

Polyethylene glycol (PEG)-based hydrogels, with variable stiffness, are widely used in tissue engineering to investigate substrate stiffness effects on cell properties. Transcriptome analysis is a critical method for understanding cell physiology. However, significant RNA degradation was observed in...

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Détails bibliographiques
Publié dans:Anal Biochem
Auteurs principaux: Gasparian, Alexander, Daneshian, Leily, Ji, Hao, Jabbari, Esmaiel, Shtutman, Michael
Format: Artigo
Langue:Inglês
Publié: 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4497926/
https://ncbi.nlm.nih.gov/pubmed/25963891
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.05.002
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