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Determination of concentration and activity of immobilized enzymes

Methods that directly measure the concentration of surface-immobilized biomolecules are scarce. More commonly the concentration of the soluble molecule is measured before and after immobilization, and the bound concentration is assessed by elimination, assuming that all bound molecules are active. A...

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Detalhes bibliográficos
Publicado no:Anal Biochem
Main Authors: Singh, Priyanka, Morris, Holly, Tivanski, Alexei V., Kohen, Amnon
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4497861/
https://ncbi.nlm.nih.gov/pubmed/25707319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.02.014
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