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Expression and Characterization of the RKOD DNA Polymerase in Pichia pastoris
The present study assessed high-level expression of the KOD DNA polymerase in Pichia pastoris. Thermococcus kodakaraensis KOD1 is a DNA polymerase that is widely used in PCR. The DNA coding sequence of KOD was optimized based on the codon usage bias of P. pastoris and synthesized by overlapping PCR,...
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Gepubliceerd in: | PLoS One |
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Hoofdauteurs: | , , , , , , , , |
Formaat: | Artigo |
Taal: | Inglês |
Gepubliceerd in: |
Public Library of Science
2015
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Onderwerpen: | |
Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4489709/ https://ncbi.nlm.nih.gov/pubmed/26134129 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0131757 |
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