Expression and Characterization of the RKOD DNA Polymerase in Pichia pastoris

The present study assessed high-level expression of the KOD DNA polymerase in Pichia pastoris. Thermococcus kodakaraensis KOD1 is a DNA polymerase that is widely used in PCR. The DNA coding sequence of KOD was optimized based on the codon usage bias of P. pastoris and synthesized by overlapping PCR,...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Pubblicato in:PLoS One
Autori principali: Wang, Fei, Li, Shuntang, Zhao, Hui, Bian, Lu, Chen, Liang, Zhang, Zhen, Zhong, Xing, Ma, Lixin, Yu, Xiaolan
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2015
Soggetti:
Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4489709/
https://ncbi.nlm.nih.gov/pubmed/26134129
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0131757
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !

Documenti analoghi