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Optimizing fluorescent protein trios for 3-Way FRET imaging of protein interactions in living cells

Powerful new methods have extended FRET microscopy to the imaging of three or more interacting proteins inside living cells. Here, we compared widely available fluorescent proteins to find the best trio for 3-Way FRET imaging. We focused on readily available cyan, yellow, and red proteins that have...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Scott, Brandon L., Hoppe, Adam D.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4487001/
https://ncbi.nlm.nih.gov/pubmed/26130463
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep10270
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