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Creation of Primary Cell Lines from Lineage-Labeled Mouse Models of Cancer
Frequently, it is necessary to isolate pure populations of cancer cells for downstream assays, such as transcriptional analysis, signaling studies, and the creation of noncontaminated primary cell lines. Genetic lineage labeling with fluorescent reporter alleles allows for the identification of epit...
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Publicado no: | Cold Spring Harb Protoc |
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Autor principal: | |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2015
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4483171/ https://ncbi.nlm.nih.gov/pubmed/25934932 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot078287 |
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