Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes

Here, we introduce a new serum-free defined medium (SPM) that supports the cultivation of human pluripotent stem cells (hPSCs) on recombinant human vitronectin-N (rhVNT-N)-coated dishes after seeding with either cell clumps or single cells. With this system, there was no need for an intervening sequ...

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
में प्रकाशित:PLoS One
मुख्य लेखकों: Takenaka, Chiemi, Miyajima, Hiroshi, Yoda, Yusuke, Imazato, Hideo, Yamamoto, Takako, Gomi, Shinichi, Ohshima, Yasuhiro, Kagawa, Kenichi, Sasaki, Tetsuji, Kawamata, Shin
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: Public Library of Science 2015
विषय:
Research Article
ऑनलाइन पहुंच:https://ncbi.nlm.nih.gov/pmc/articles/PMC4483150/
https://ncbi.nlm.nih.gov/pubmed/26115194
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0129855
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