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Universal Approach to FRAP Analysis of Arbitrary Bleaching Patterns

The original approach to calculating diffusion coefficients of a fluorescent probe from Fluorescence Recovery After Photobleaching (FRAP) measurements assumes bleaching with a circular laser beam of a Gaussian intensity profile. This method was used without imaging the bleached cell. An empirical eq...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Blumenthal, Daniel, Goldstien, Leo, Edidin, Michael, Gheber, Levi A.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4479983/
https://ncbi.nlm.nih.gov/pubmed/26108191
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep11655
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