Quantitative lipopolysaccharide analysis using HPLC/MS/MS and its combination with the limulus amebocyte lysate assay

Quantitation of plasma lipopolysaccharides (LPSs) might be used to document Gram-negative bacterial infection. In the present work, LPS-derived 3-hydroxymyristate was extracted from plasma samples with an organic solvent, separated by reversed phase HPLC, and quantitated by MS/MS. This mass assay wa...

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Detalhes bibliográficos
Publicado no:J Lipid Res
Main Authors: Pais de Barros, Jean-Paul, Gautier, Thomas, Sali, Wahib, Adrie, Christophe, Choubley, Hélène, Charron, Emilie, Lalande, Caroline, Le Guern, Naig, Deckert, Valérie, Monchi, Mehran, Quenot, Jean-Pierre, Lagrost, Laurent
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2015
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4479340/
https://ncbi.nlm.nih.gov/pubmed/26023073
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D059725
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