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3D-Time-Lapse Analysis of Rab11/FIP5 Complex: Spatiotemporal Dynamics during Apical Lumen Formation

Fluorescent imaging of fixed cells grown in two-dimensional (2D) cultures is one of the most widely used techniques for observing protein localization and distribution within cells. Although this technique can also be applied to polarized epithelial cells that form three-dimensional (3D) cysts when...

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Bibliografiske detaljer
Udgivet i:Methods Mol Biol
Main Authors: Mangan, Anthony, Prekeris, Rytis
Format: Artigo
Sprog:Inglês
Udgivet: 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4479190/
https://ncbi.nlm.nih.gov/pubmed/25800842
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2569-8_15
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