Optimized Protocol for Simple Extraction of High-Quality Genomic DNA from Clostridium difficile for Whole-Genome Sequencing

Successful sequencing of the Clostridium difficile genome requires high-quality genomic DNA (gDNA) as the starting material. gDNA extraction using conventional methods is laborious. We describe here an optimized method for the simple extraction of C. difficile gDNA using the QIAamp DNA minikit, whic...

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書目詳細資料
發表在:J Clin Microbiol
Main Authors: Sim, James Heng Chiak, Anikst, Victoria, Lohith, Akshar, Pourmand, Nader, Banaei, Niaz
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2015
主題:
Bacteriology
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4473243/
https://ncbi.nlm.nih.gov/pubmed/25878343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00956-15
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