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Rapid preparation of rodent testicular cell suspensions and spermatogenic stages purification by flow cytometry using a novel blue-laser-excitable vital dye

Availability of purified or highly enriched fractions representing the various spermatogenic stages is a usual requirement to study mammalian spermatogenesis at the molecular level. Fast preparation of high quality testicular cell suspensions is crucial when flow cytometry (FCM) is chosen to accompl...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: Rodríguez-Casuriaga, Rosana, Santiñaque, Federico F., Folle, Gustavo A., Souza, Elisa, López-Carro, Beatriz, Geisinger, Adriana
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4473029/
https://ncbi.nlm.nih.gov/pubmed/26150958
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2014.10.002
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