Comparative analysis and validation of the malachite green assay for the high throughput biochemical characterization of terpene synthases

Terpenes are the largest group of natural products with important and diverse biological roles, while of tremendous economic value as fragrances, flavours and pharmaceutical agents. Class-I terpene synthases (TPSs), the dominant type of TPS enzymes, catalyze the conversion of prenyl diphosphates to...

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Bibliographic Details
Published in:MethodsX
Main Authors: Vardakou, Maria, Salmon, Melissa, Faraldos, Juan A., O’Maille, Paul E.
Format: Artigo
Language:Inglês
Published: Elsevier 2014
Subjects:
Biochemistry, Genetics and Molecular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4472957/
https://ncbi.nlm.nih.gov/pubmed/26150952
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2014.08.007
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