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A Malachite Green-Based Assay to Assess Glucan Phosphatase Activity

With the recent discovery of a unique class of dual-specificity phosphatases that dephosphorylate glucans, we report an in vitro assay tailored for the detection of phosphatase activity against phosphorylated glucans. We demonstrate that in contrast to a general phosphatase assay utilizing a synthet...

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Detalhes bibliográficos
Main Authors: Sherwood, Amanda R., Paasch, Bradley C., Worby, Carolyn A., Gentry, Matthew S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3577954/
https://ncbi.nlm.nih.gov/pubmed/23201267
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.10.044
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