Live-cell multiphoton fluorescence correlation spectroscopy with an improved large Stokes shift fluorescent protein

We report an improved variant of mKeima, a monomeric long Stokes shift red fluorescent protein, hmKeima8.5. The increased intracellular brightness and large Stokes shift (∼180 nm) make it an excellent partner with teal fluorescent protein (mTFP1) for multiphoton, multicolor applications. Excitation...

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Publié dans:Mol Biol Cell
Auteurs principaux: Guan, Yinghua, Meurer, Matthias, Raghavan, Sarada, Rebane, Aleksander, Lindquist, Jake R., Santos, Sofia, Kats, Ilia, Davidson, Michael W., Mazitschek, Ralph, Hughes, Thomas E., Drobizhev, Mikhail, Knop, Michael, Shah, Jagesh V.
Format: Artigo
Langue:Inglês
Publié: The American Society for Cell Biology 2015
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Articles
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4472016/
https://ncbi.nlm.nih.gov/pubmed/25877871
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E14-10-1473
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