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Luciferase Protein Complementation Assays for Bioluminescence Imaging of Cells and Mice
Protein fragment complementation assays (PCAs) with luciferase reporters currently are the preferred method for detecting and quantifying protein-protein interactions in living animals. At the most basic level, PCAs involve fusion of two proteins of interest to enzymatically inactive fragments of lu...
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| 發表在: | Methods Mol Biol |
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| Main Authors: | , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2011
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4467521/ https://ncbi.nlm.nih.gov/pubmed/21153371 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60761-901-7_2 |
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