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RiboTag is a flexible tool for measuring the translational state of targeted cells in heterogeneous cell cultures

Primary neuronal cultures are a useful tool for measuring pharmacological- and transgene-regulated gene expression; however, accurate measurements can be confounded by heterogeneous cell-types and inconsistent transfection efficiency. Here we describe our adaptation of a ribosomal capture strategy t...

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Foilsithe in:Biotechniques
Main Authors: Lesiak, Adam J., Brodsky, Matthew, Neumaier, John F.
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2015
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4467021/
https://ncbi.nlm.nih.gov/pubmed/26054767
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114299
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