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Production and characterization of thirteen human Type-I interferon-α subtypes
Thirteen human interferon-α (IFNα) subtypes were expressed in E. coli and purified using an N-terminal affinity tag from the prodomain of subtilisn. IFNα subtypes were expressed in soluble form and purified from cell lysates or refolded and purified from inclusion bodies. Proteins produced by either...
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| 出版年: | Protein Expr Purif |
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| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2014
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4466898/ https://ncbi.nlm.nih.gov/pubmed/25149396 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2014.08.010 |
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