Immunocapture and microplate-based activity and quantity measurement of pyruvate dehydrogenase in human peripheral blood mononuclear cells

BACKGROUND: Pyruvate dehydrogenase (PDH) activity is altered in many human disorders. Current methods require tissue samples and yield inconsistent results. We describe a modified method for measuring PDH activity from isolated human peripheral blood mononuclear cells (PBMCs). RESULTS/METHODOLOGY: W...

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Bibliografske podrobnosti
izdano v:Bioanalysis
Main Authors: Liu, Xiaowen, Pervez, Hira, Andersen, Lars W, Uber, Amy, Montissol, Sophia, Patel, Parth, Donnino, Michael W
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4465223/
https://ncbi.nlm.nih.gov/pubmed/25826140
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4155/bio.14.302
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