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In Situ Quantification of Protein Binding to the Plasma Membrane
This study presents a fluorescence-based assay that allows for direct measurement of protein binding to the plasma membrane inside living cells. An axial scan through the cell generates a fluorescence intensity profile that is analyzed to determine the membrane-bound and cytoplasmic concentrations o...
Gespeichert in:
Veröffentlicht in: | Biophys J |
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Hauptverfasser: | , , , |
Format: | Artigo |
Sprache: | Inglês |
Veröffentlicht: |
The Biophysical Society
2015
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Schlagworte: | |
Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4457494/ https://ncbi.nlm.nih.gov/pubmed/26039166 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.04.021 |
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