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In Situ Quantification of Protein Binding to the Plasma Membrane

This study presents a fluorescence-based assay that allows for direct measurement of protein binding to the plasma membrane inside living cells. An axial scan through the cell generates a fluorescence intensity profile that is analyzed to determine the membrane-bound and cytoplasmic concentrations o...

詳細記述

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書誌詳細
出版年:Biophys J
主要な著者: Smith, Elizabeth M., Hennen, Jared, Chen, Yan, Mueller, Joachim D.
フォーマット: Artigo
言語:Inglês
出版事項: The Biophysical Society 2015
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC4457494/
https://ncbi.nlm.nih.gov/pubmed/26039166
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.04.021
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