Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

For more than 20 years, reverse pharmacology has been the preeminent strategy to discover the activating ligands of orphan G protein-coupled receptors (GPCRs). The onset of a reverse pharmacology assay is the cloning and subsequent transfection of a GPCR of interest in a cellular expression system....

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Detaylı Bibliyografya
Yayımlandı:J Vis Exp
Asıl Yazarlar: Caers, Jelle, Peymen, Katleen, Suetens, Nick, Temmerman, Liesbet, Janssen, Tom, Schoofs, Liliane, Beets, Isabel
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MyJove Corporation 2014
Konular:
Cellular Biology
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4457351/
https://ncbi.nlm.nih.gov/pubmed/25146596
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51516
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