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Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
For more than 20 years, reverse pharmacology has been the preeminent strategy to discover the activating ligands of orphan G protein-coupled receptors (GPCRs). The onset of a reverse pharmacology assay is the cloning and subsequent transfection of a GPCR of interest in a cellular expression system....
Sparad:
| I publikationen: | J Vis Exp |
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| Huvudupphovsmän: | , , , , , , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
MyJove Corporation
2014
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4457351/ https://ncbi.nlm.nih.gov/pubmed/25146596 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51516 |
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