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Efficient Soluble Expression of Active Recombinant Human Cyclin A2 Mediated by E. coli Molecular Chaperones

Bacterial expression of human proteins continues to present a critical challenge in protein crystallography and drug design. While human cyclin A constructs have been extensively characterized in complex with cyclin dependent kinase 2 (CDK2), efforts to express the monomeric human cyclin A2 in Esche...

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Detalhes bibliográficos
Publicado no:Protein Expr Purif
Main Authors: Grigoroudis, Asterios I., McInnes, Campbell, Premnath, Padmavathy Nandha, Kontopidis, George
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4456239/
https://ncbi.nlm.nih.gov/pubmed/25956535
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2015.01.013
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