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Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2

Our understanding of the genus Natrinema is presently limited due to the lack of available genetic tools. Auxotrophic markers have been widely used to construct genetic systems in bacteria and eukaryotes and in some archaeal species. Here, we isolated four auxotrophic mutants of Natrinema sp. J7-2,...

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Publicat a:Archaea
Autors principals: Lv, Jie, Wang, Shuai, Wang, Yuchen, Huang, Yuping, Chen, Xiangdong
Format: Artigo
Idioma:Inglês
Publicat: Hindawi Publishing Corporation 2015
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4454726/
https://ncbi.nlm.nih.gov/pubmed/26089742
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2015/483194
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