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Removal of Out-of-Plane Fluorescence for Single Cell Visualization and Quantification in Cryo-Imaging

We developed a cryo-imaging system, which alternates between sectioning (10–40 μm) and imaging bright field and fluorescence block-face image volumes with micronscale-resolution. For applications requiring single-cell detection of fluorescently labeled cells anywhere in a mouse, we are developing so...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Ann Biomed Eng
Hauptverfasser: Steyer, Grant J., Roy, Debashish, Salvado, Olivier, Stone, Meredith E., Wilson, David L.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4452119/
https://ncbi.nlm.nih.gov/pubmed/19513848
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10439-009-9726-x
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