Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells

BACKGROUND: Expression vector engineering technology is one of the most convenient and timely method for cell line development to meet the rising demand of novel production cell line with high productivity. Destabilization of dihydrofolate reductase (dhfr) selection marker by addition of AU-rich ele...

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Publicado en:BMC Biotechnol
Autores principales: Chin, Christine Lin, Chin, Hing Kah, Chin, Cara Sze Hui, Lai, Ethan Tingfeng, Ng, Say Kong
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2015
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Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4450478/
https://ncbi.nlm.nih.gov/pubmed/26033090
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-015-0145-9
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