Simultaneous Discrimination between 15 Fish Pathogens by Using 16S Ribosomal DNA PCR and DNA Microarrays

We developed a DNA microarray suitable for simultaneous detection and discrimination between multiple bacterial species based on 16S ribosomal DNA (rDNA) polymorphisms using glass slides. Microarray probes (22- to 31-mer oligonucleotides) were spotted onto Teflon-masked, epoxy-silane-derivatized gla...

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Detalhes bibliográficos
Main Authors: Warsen, Adelaide E., Krug, Melissa J., LaFrentz, Stacey, Stanek, Danielle R., Loge, Frank J., Call, Douglas R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC444826/
https://ncbi.nlm.nih.gov/pubmed/15240304
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.7.4216-4221.2004
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