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Enzymatic Synthesis of High-Molecular-Mass Poly-γ-Glutamate and Regulation of Its Stereochemistry

For the first time, we succeeded in synthesizing in vitro poly-γ-glutamate (PGA) with high molecular masses (>1,000 kDa) by the use of enzyme-associated cell membranes from Bacillus subtilis subsp. chungkookjang. The activity for PGA synthesis, however, was readily lost in the presence of critica...

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Detalhes bibliográficos
Main Authors: Ashiuchi, Makoto, Shimanouchi, Kazuya, Nakamura, Hisaaki, Kamei, Tohru, Soda, Kenji, Park, Chung, Sung, Moon-Hee, Misono, Haruo
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC444813/
https://ncbi.nlm.nih.gov/pubmed/15240308
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.7.4249-4255.2004
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