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New Real-Time Quantitative PCR Procedure for Quantification of Bifidobacteria in Human Fecal Samples

The application of a real-time quantitative PCR method (5′ nuclease assay), based on the use of a probe labeled at its 5′ end with a stable, fluorescent lanthanide chelate, for the quantification of human fecal bifidobacteria was evaluated. The specificities of the primers and the primer-probe combi...

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Detalles Bibliográficos
Main Authors: Gueimonde, Miguel, Tölkkö, Satu, Korpimäki, Teemu, Salminen, Seppo
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2004
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC444799/
https://ncbi.nlm.nih.gov/pubmed/15240297
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.7.4165-4169.2004
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