Combined Immunomagnetic Separation-Molecular Beacon-Reverse Transcription-PCR Assay for Detection of Hepatitis A Virus from Environmental Samples

In this study, a molecular-beacon-based real-time reverse transcription (RT)-PCR assay was developed to detect the presence of hepatitis A virus (HAV) in environmental samples. A 125-bp, highly conserved 5′ noncoding region of HAV was targeted. The sensitivity of the real-time RT-PCR assay was teste...

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Detalhes bibliográficos
Main Authors: Abd El Galil, Khaled H., El Sokkary, M. A., Kheira, S. M., Salazar, Andre M., Yates, Marylynn V., Chen, Wilfred, Mulchandani, Ashok
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC444796/
https://ncbi.nlm.nih.gov/pubmed/15240322
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.7.4371-4374.2004
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