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MEASUREMENT OF LIPOLYSIS PRODUCTS SECRETED BY 3T3-L1 ADIPOCYTES USING MICROFLUIDICS

Glass microfluidic devices have been fabricated to monitor the secretion of glycerol or fatty acids from cultured murine 3T3-L1 adipocytes. In the current studies, adipocytes are perfused in a reversibly-sealed cell chamber and secreted products are analyzed by enzyme assay on either a single- or du...

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Detalhes bibliográficos
Publicado no:Methods Enzymol
Main Authors: Dugan, Colleen E., Kennedy, Robert T.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4440587/
https://ncbi.nlm.nih.gov/pubmed/24529440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-800280-3.00011-6
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